The following are affiliated unions with the Lee County Labor Chapter:
AFSCME LOCAL 2005
AFSCME LOCAL 2205
AFSCME LOCAL 2989
AFSCME LOCAL 3929
BCTGM Local 48G
BLET LOCAL 391
HFIAW LOCAL 81
IAFF LOCAL 568
IAM Local 1010
IBEW LOCAL 13
IBEW LOCAL 204
IUPAT-PPME LOCAL 2003
NALC LOCAL 403
OPEIU LOCAL 277
UA LOCAL 125
UFCW LOCAL 617
USW LOCAL 9B
USW LOCAL 444